Understand the meaning of effect-directedanalysis as well as advantages and disadvantagesof the different techniques
Survey the variety of planar assays (on surface,in situ, in the adsorbent bed)
Know the streamlined workflow on one plate, i.e. parallel separation of compounds in complex samples, discovery of active compounds and theircharacterization by chromatographic, spectroscopic and spectrometric information(3-20 min/sample for up to 20 samples in parallel)
Recognize the highly efficient combination ofplanar chromatography with biological andbiochemical or other effect-directed assays
Realize the power of hyphenated HPTLC and theunique benefit of effect-directed profiles

Antimicrobials via Gram-negative Aliivibrio fischeriand Gram-positive Bacillus subtilis bioassays
Enzyme inhibitors via α- and ß-glucosidase,α-amylase, acetyl- and butyrylcholinesterase,tyrosinase and ß-glucuronidase assays
Genotoxic compounds via SOS-Umu-C bioassay,cytotoxic bioassay, neurotoxic bioassay
Endocrine-acting compounds via planar yeastestrogen/androgen screen (pYES/pYAS)
Multiplex planar bioassays: agonistic andantagonistic effect detection (pYAES/pYAAS) with verification (pYAVES/pYAVAS) and detection ofsynergists → 4-fold multiplex
nanoGIT+active: on-surface simulated static digestivesystem including separation and effect detection
On-surface metabolization via S9 liver enzymesystem
On-surface adhesive/adherent cell assays
Coupling to HESI-HRMS and DART-MS

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