Hyphenated HPTLC

The participants

• Understand the meaning of effect-directed analysis as well as advantages and disadvantagesof the different techniques
• Survey the variety of planar assays (on surface,in situ, in the adsorbent bed)
• Know the streamlined workflow on one plate, i.e. parallel separation of compounds in complex samples, discovery of active compounds and their characterization by chromatographic, spectroscopic and spectrometric information (3-20 min/sample for up to 20 samples in parallel)
• Recognize the highly efficient combination of planar chromatography with biological andbiochemical or other effect-directed assays
• Realize the power of hyphenated HPTLC and the unique benefit of effect-directed profiles

Content

• Antimicrobials via Gram-negative Aliivibrio fischeri and Gram-positive Bacillus subtilis bioassays
• Enzyme inhibitors via α- and ß-glucosidase,α-amylase, acetyl- and butyrylcholinesterase, tyrosinase and ß-glucuronidase assays
• Genotoxic compounds via SOS-Umu-C bioassay,cytotoxic bioassay, neurotoxic bioassay
• Endocrine-acting compounds via planar yeastestrogen/androgen screen (pYES/pYAS)
• Multiplex planar bioassays: agonistic andantagonistic effect detection (pYAES/pYAAS) with verification (pYAVES/pYAVAS) and detection ofsynergists → 4-fold multiplex
• nanoGIT+active: on-surface simulated static digestivesystem including separation and effect detection
• On-surface metabolization via S9 liver enzymesystem
• On-surface adhesive/adherent cell assays
• Coupling to HESI-HRMS and DART-MS

• Scientists in the field of consumer protection, analytical/environmental/medical chemistry, forensic, doping etc.
• Food chemists and analysts in official labs (to learn the powerful priorization strategy)
• Producing industries (to learn more about hazards in products)
• University staff (to teach on sophisticated HPTLC-EDA)
• Researcher in all fields (to better understand complex samples)