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Highlights

Chromatography combined with planar biological/biochemical assays

Pointing to single bioactive/hazardous compounds in complex samples

Fast toxicological profiling of samples and prioritization on active compounds

High-performance thin-layer chromatography combined with effect-directed assays

Recording of high-resolution mass spectra from active compound zones

Goals & Content


    The participants

    • Understand the meaning of effect-directed analysis as well as advantages and disadvantages of the different techniques
    • Survey the variety of planar assays (on surface, in situ, in the adsorbent bed)
    • Know the streamlined workflow on one plate, i.e. parallel separation of compounds in complex samples, discovery of active compounds and their characterization by chromatographic, spectroscopic and spectrometric information (3-20 min/sample for up to 20 samples in parallel)
    • Recognize the highly efficient combination of planar chromatography with biological and biochemical or other effect-directed assays
    • Realize the power of hyphenated HPTLC and the unique benefit of effect-directed profiles

    Content

    • Antimicrobials via Gram-negative Aliivibrio fischeri and Gram-positive Bacillus subtilis bioassays
    • Enzyme inhibitors via α- and ß-glucosidase, α-amylase, acetyl- and butyrylcholinesterase, tyrosinase and ß-glucuronidase assays
    • Genotoxic compounds via SOS-Umu-C bioassay, cytotoxic bioassay, neurotoxic bioassay
    • Endocrine-acting compounds via planar yeast estrogen/androgen screen (pYES/pYAS)
    • Multiplex planar bioassays: agonistic and antagonistic effect detection (pYAES/pYAAS) with verification (pYAVES/pYAVAS) and detection of synergists → 4-fold multiplex
    • NanoGIT+active: on-surface simulated static digestive system including separation and effect detection
    • On-surface metabolization via S9 liver enzyme system
    • On-surface adhesive/adherent cell assays
    • Coupling to HESI-HRMS and DART-MS

    About the Course Management

    Prof. Dr. Gertrud Morlock

    Justus-Liebig-Universität Gießen

    Prof. Dr. Gertrud Morlock

    Justus-Liebig-Universität Gießen

    Target Group


    • Scientists in the field of consumer protection, analytical/environmental/medical chemistry, forensic, doping etc.
    • Food chemists and analysts in official labs (to learn the powerful priorization strategy)
    • Producing industries (to learn more about hazards in products)
    • University staff (to teach on sophisticated HPTLC-EDA)
    • Researcher in all fields (to better understand complex samples) 

    Contact

    Any questions or need for information?

    We are happy to hear from you.

    Contact

    Any questions or need for information?

    We are happy to hear from you.